Assay chromatin fertility sperm sperm structure test
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Many of those couples who have been previously unable to conceive with what would be considered extreme measures have been diagnosed with high sperm DNA fragmentation and treated. More importantly, what has also been demonstrated is that the degree of DNA fragmentation correlates very highly with the inability of the sperm to initiate a birth regardless of the technology used to fertilize the egg such as insemination, IVF or ICSI. It is akin to seeing the tip of the iceberg. It is a completely independent variable.
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Dna Fragmentation In Spermatozoa
The sperm chromatin structure assay SCSA is a new diagnostic tool that can detect sperm samples that have a high degree of DNA fragmentation small breaks in the sperm chromosomes. Simply put, DNA is arranged in a double helix or ladder configuration with side rails and rungs. This test uses special stains and complex instrumentation flow cytometer , and a laser beam to quantitate the percentage of sperm with intact vs. The integrity of mammalian sperm DNA is of prime importance for the paternal genetic contribution to normal offspring. It is very important to understand that sperm DNA fragmentation can change with time and it can be improved in many cases. It is now very clear to see that having this information about the quality of the sperm can be tremendously helpful to couples and their physicians.
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What is sperm chromatin structure assay
About a year and a half ago a relatively new concept was introduced to clinical practice. sperm quality was dependent on the amount of damage to the sperm DNA or DNA fragmentation. Those couples using egg donors or surrogates may also benefit from screening prior to going thru the procedures because the effort and costs are so great. Spermatazoa with high DNA fragmentation may fertilize an egg and embryo development stops before implantation or may even initiate a pregnancy, but there is a significantly higher likelihood that it will result in miscarriage. The sperm are then labeled with a special orange colored dye that only attaches to the ends of broken DNA within the sperm cell. The integrity of mammalian sperm DNA is of prime importance for the paternal genetic contribution to normal offspring. This comprehensive and detailed unit presents a rapid, reliable, practical test for DNA integrity based on staining with acridine orange.
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